摘要
以大白鼠脑组织作催化鸟嘌呤脱氨反应的酶源,采用氨气敏电极为基础电极制成测定鸟嘌呤的大白鼠脑组织电极,对其响应特性、介质条件、固定化方法等因素进行了研究,测定鸟嘌呤的E～log[鸟嘌呤]曲线斜率为46.5mV,线性范围2.0×10^(-5)～6.3×10^(-4)mol/L,寿命可达28d。
An electrode for the determination of guanine was prepared, which employed brain tissue of rat as the biocatalyst and an ammonia gas sensing electrode as the base electrone. Its response characteristics, buffer conditions and strategies of immobilization were studied. The slope of guanine response curve E-lg[Guanine] is 46. 5 mV/decade, linear range is 2. 0 × 10-5-6. 3×10-4 mol/L, lifetime is over 28 days.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1992年第5期583-585,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
长春应用化学研究所电分析化学开放实验室资助
关键词
鸟嘌呤
组织电级
生物传感器
脑
Brain tissue of rat, Guanine, Tissue based electrode, Biosensor
