光化学-荧光分析法研究(Ⅷ)——波长扫描-反应速差分析法

Studies on Photochemical Fluorimetric Method(Ⅷ)——Wave Scanning-Differential Rate Method

提出了波长扫描-光化学反应速差荧光分析同时测定双组分的方法,以核黄素的光分解反应和甲萘醌的光还原反应为例考察了方法的可行性.结果表明,核黄素、甲萘醌浓度分别在(0～1.20)μg/mL和(0～2.70)μg/mL范围内呈良好的线性关系(γ为0.999和0.998).检出限分别为0.22ng/mL和2.6 ng/mL(n=11).平均回收率分别为101％、99％.

The present paper deals with a differential rate kinetic photochemical fluorimetric technique,wave scanning-differential rate method,for simultaneous determination of two compounds.The principle for this method as well as the experimental technique by which the in situ photochemical fluorimetry is able to carry out by use of commercial fluorescence spectropho-tometer is described.A wave scanning-differential rate method for the simultaneous determination of riboflavin and menadione based on their photochemical reaction,used as an example of this technique,is proposed,The calibration graph was rectilinear from 0.0 ng/mL to 1.20μg/mL for riboflavin (r=0.999) and from 0.0 ng/mL to 2.70 /ng/mL for menadione (r=0.998).The limits of detection were 0.22 ng/mL riboflavin and 2.6 ng/mL menadione.The average recoveries were 101% and 99%,respectively.The standard deviations were 2.5% for riboflavin and 2.8% for menadione