毛细管凝胶电泳的激光光热在线检测

Laser Opto-thermal On-line Detection for the Gel Capillary Electrophoresis

<正> 近年来,毛细管电泳已成为一种高效快速的微化学分离分析新技术,在无机、有机及生物化学分析中得到了广泛的应用.但毛细管的内径小,进样量在pg～ng级,给样品的检测带来新的困难.因此,发展高灵敏度的显微在线检测器,已成为毛细管电泳技术发展中的重要环节.基于各种原理的检测器研究已有报道~[1].Dovichi等曾用热透镜技术在线检测了乙腈/

The opto-thermal detection technique based on crossed-beam thermal lens effect has been developed for the on-line determination of capillary polyacrylamide gel electrophoresis(PAGE).The minimum detectable concentration of methylene blue in ethanol filled into 220 pm i.d.capillary was obtained to be 1.76×10-9 mol/L,corresponding to the absorbance of 3.8×10-6.A mixture of bromophenol blue and amide black was separated by capillary PAGE and detected by laser opto-thermal on-line detection technique.Mass detection limit for bromophenol blue injected into the gel capillary was found to be 40 pg.