丹参酮Ⅱ_A疏水相互作用的电镜观察

Observation on the hydrophobic interaction of TanshinoneⅡ_A by transmission electron microscope

借助透射电镜观察丹参酮ⅡA 在不同比例的Ringer’s生理盐水条件下形成胶体颗粒的过程 ,得到以下结果 :1)丹参酮结晶粉末分散在甲醇后的一段时间内仍然以结晶状态存在 ,与其处于固体状态下相比结晶的尺寸减小到微米数量级。 2 )Ringer’s生理盐水的比例对丹参酮结晶的分散速度和胶粒的形成具有直接的影响 ,比例增大能加快丹参酮结晶的分散速度和胶粒的形成。 3 )在分散介质中的呈棒状的丹参酮结晶颗粒在其宽度处于 5 0～ 60nm时将不再分解成更小的结晶颗粒 ,而是开始失去结晶特有的形态 ,逐渐的被分散形成直径小于 5nm的微胶粒并积聚成团块。在团块中的微胶粒进而积聚形成直径约为 2 0nm的胶粒。 4)直径约为 5 0nm胶粒是由直径约为 2 0nm的胶粒积聚成的团块形成的 ,其特征是彼此挨个相连接成链状并有缠绕结构形态 ,个体的特征是边缘清晰 ,大小规则 ,呈椭球形 ,直径约为 5 0nm ,椭球的长轴是沿着胶粒彼此相连的方向。 5 )丹参酮在分散相中所能保持的最小结晶颗粒的宽度是 5 0～ 60nm ,而形成的胶粒直径也是 5 0～ 60nm。

TanshinoneⅡ A is one of the physiologically active constituents of Dan shen (Salvia miltiorrhiza)and belongs to lipoid quinone. It can be transformed into colloid by the driving force of water media. In our experiment, the formation process of colloid from TanshinoneⅡ A by adding an aliquot of the methanol stock solution of TanshinoneⅡ A into a serial propotion of Ringer's solution were observed at an interval of 5 day for 10 days by TEM. The results show that the concentration of Ringer's solution affects directly the dimensional distribution of crystal TanshinoneⅡ A and the speed of development of colloidal particles of TanshinoneⅡ A. The crystal, when 50～60nm in width, disperses into colloidal particles instead of smaller crystal particles. The colloidal particles were initially less than 5nm in diameter but gradually condensed into clusters with maximum size of 50～60nm. It infers that the smallest width of crystal might be related with the diameter of colloidal particles developed finally in water media.