柞蚕蛹皮和桑蚕蛹皮的扫描电镜比较观察

Comparison of structures between Antheraea pernyi crust and Bombyx mori crust by SEM

将柞蚕蛹皮和桑蚕蛹皮分别经过脱脂、去无机盐、去蛋白质等处理 ,并用扫描电镜进行比较观察。结果表明 ,经不同处理的蛹皮在内、外表面形貌 ,几丁质、气门的结构 ,以及有无刚毛上差别很大。但在蛋白质、几丁质和无机盐三者的存在方式上两者基本相同 ,即几丁质与蛋白质形成复合体并构成蛹皮的壳骨架 ,颗粒状无机盐均匀分布在该复合体的内部 ,均呈平行于蛹皮表面的层状分布。

Antheraea pernyi crust and Bombyx mori crust were treated by defatting, removing inorganic salts and deproteinizing respectively, and observed by SEM. The results show that they are quite different in several aspects such as the inner, outer surface, chitin and spiracle structure, and with or without sela etc., but nearly the same in their existing pattern of protein, chitin and inorganic salts. That is, the chitin protein complex forms the pupa crust skeleton, and the inorganic salts like particles distribute regularly inside the complex. The skeleton is a layer like structure which is parallel with the surface of pupa crust. However, there is no detailed report yet about the surface microstructure of two kinds of pupa crusts. So the present research could provide an experimental evidence for working out the way of isolating chitin from pupa crust.