淋巴瘤细针吸取细胞学与组织学及免疫组化对照研究

Control study of cytology,histopathology and immunohistochemistry of lymphomas in fine-needle aspiration cytology

目的 :研究淋巴瘤细针吸取细胞学 (FNAC)的形态特征及其在诊断中的价值。 方法 :对 2 7例淋巴瘤进行细针吸取细胞学及组织学检查 ,并运用免疫组化ABC法检测 ,3例作透射电镜观察。 结果 :2 7例淋巴瘤中 ,细胞学确定诊断淋巴瘤 12例 ,霍奇金淋巴瘤 3例 ,分类诊断准确率为 5 5 .6 % (15 / 2 7) ;细胞学高度怀疑淋巴瘤 8例 ,均经组织学证实为淋巴瘤 ,细胞学分类诊断符合率为 85 .2 % (2 3/ 2 7) ;细胞学诊断转移性癌 3例 ,2例经组织病理检查诊断为T细胞性淋巴瘤 ,1例为B细胞性淋巴瘤。细胞学诊断反应性增生 1例 ,组织学证实为B细胞性淋巴瘤。全部病例诊断阳性率 96 .3% (2 6 / 2 7)。 结论 :针吸细胞学检查对初筛淋巴结疾患的良恶性及肿瘤类型有重要意义。组织学检查能确定肿瘤的性质、组织起源与分类。

Objectives:To investigate the morphological features of lymphomas by fine needle aspiration biopsy(FNAC) and its value in diagnosis. Methods:Twenty seven cases of lymphomas were examined by FNAC and histopathology with immunohistochemistry.Three cases were studied by transmission electron microscope. Results:In 27 cases of lymphomas,12 cases were diagnosed as lymphoma by cytological examination and 3 cases were suspected as lymphoma, which were confirmed by histopathology.The total consistent rate of cytopathology diagnosis with histopathology classfication were 85.2%(23/27).3 cases were misdiagnosed as metastasis carcinoma by cytopathology,among which 2 cases were confirmed as T cell lymphoma by histopathology and the other was B cell lymphoma.One case was diagnosed as reactive hyperplasia by cytopathology,which was confirmed as B cell lymphoma by histopathology.The total positive rates of all cases were 96.3%(26/27). Conclusions:FNAC is very important to the first screening in the benign malignant lymph node lesions and tumor type.Histopathology can confirmed tumor malignancy,tissue origin and classification.Immunohistochemistry is of great help in the classification of lymphoma and final diagnosis.