摘要
目的建立26个多态性STR标记,进行克隆牛的鉴定。方法采用荧光标记PCR引物,进行PCR扩增,应用GeneScan技术分析26个牛的微卫星基因座遗传多态性。结果26对荧光标记引物均能对上述样品很好地扩增出DNA产物,并得到清晰的条带。根据实验观察到的数据计算,体细胞克隆牛供体奶牛与克隆小奶牛的偶合率为3.31×10-15,认定它们为相同来源;在所检测的26个微卫星标记中,体细胞克隆牛供体奶牛、克隆小奶牛与克隆小奶牛代孕荷斯坦母亲的基因型在21个标记上不符合母子遗传关系,可以排除其亲子关系。结论有助于今后开展对珍稀动物或家畜被盗的案件鉴定工作。
Objective To develop26polymorphic genetics markers.Methods Based on the polymorphisms of28microsatellite loci for cattle with the GeneScan techniques,the relationship among of cloned cattle from somatic cell,her pregnant mother(Hemostat cow)and a donor for cloned somatic cell was studied.Results All26loci with fluorescence labeled primers have exhibited clear and readable genotypes.According to the data obtained in the test,the occasional ratio for a cloned cattle and a donor for cloned somatic cell was3.31×10 -15 .In conclusion,a cloned cattle came from the donor’s original.On the other hand,the genotypes of the pregnant mother(Hemostat cow)of cloned cattle are completely different in21loci of total26microsatellite loci with a cloned cattle and a donor for cloned somatic cell.They have no pedigree.Condusion The method can be used for identification of rare animal and critter in criminal cases.
出处
《刑事技术》
2003年第1期26-28,共3页
Forensic Science and Technology
