重金属污染环境来源的玉米POD对Pb^(2+)的胁迫反应

Reaction of Peroxidase of Maize(Zea mays L.) from Heavy-metal Polluted Environment to the Stress of Pb^(2+)

为了探讨植物对重金属污染环境的适应是否产生体内酶系统的耐性机制及其遗传性,以重金属污染环境来源的玉米(Zea mays L.)及其易地栽种子一代(F1)为实验材料.通过酶活性测定与同工酶电泳研究了玉米过氧化物酶对重金属Pb2+的胁迫反应.结果发现,重金属污染环境来源的玉米种群材料在重金属Pb2+的胁迫条件下,与非污染环境来源的玉米种群材料相比,其过氧化物酶具有相对较高的活性,其F1保持相同的趋势;不同玉米种群材料F1过氧化物酶同工酶酶带对重金属Pb2+的胁迫反应在材料间也出现了明显的分化,同时区分出两条对重金属Pb2+胁迫反应的敏感酶带,称之为标志性酶带.

In order to study the tolerance of plants from polluted environment to heavy metals and its heredity, the analysis of enzymatic activity and isozyme electrophoresis were adopted to probe the reaction of peroxidase(POD) of maize(Zea mays L.) samples from heavy-metal polluted environment and their first filial generation (F1) to the stress of Pb2+ . The results showed that POD activity of maize samples from polluted environment was higher than that from clear environment, and their first filial generation (Fl) showed the same trend; POD isozyme differentiated remarkably among maize samples (F1) and two isozymatic bands of POD, which were sensitive to the stress of Pb2+ and were regarded as marked bands.