基因工程人-鼠嵌合抗体ch-BDI在荷瘤鼠体内外功能的研究

In vitro and in vivo functional evaluation of anti-human bladder tumor human-mouse chimeric antibody ch-BDI

目的 鉴定抗人膀胱癌人 鼠嵌合抗体ch BDI在荷瘤鼠体内外功能 ,探讨其临床应用价值。 方法 通过超滤浓缩和亲合层析 ,得到较高纯度的ch BDI,用还原法标记99mTc,体外测定了其对人膀胱癌细胞的结合能力 ,应用放射免疫显像观察了其在荷瘤鼠体内的分布。 结果 99mTc标记的ch BDI的免疫活性分数约为 76% ,体外的亲合常数约为 3 5 6× 10 9M-1,在荷瘤鼠体内能够迅速浓缩聚集于肿瘤部位。 结论 抗人膀胱癌人 鼠嵌合抗体ch BDI在体内、体外均具有良好的与人膀胱癌组织的结合活性 。

Objective To evaluate the in vitro and in vivo function of anti human bladder tumor human mouse chimeric antibody ch BDI and its future clinical application. Methods With ch BDI in high expression cell line medium, affinity chromatography was used for the purification. Labeled with 99m Tc through reduction method, its immunoreactive fraction and as sociation constant were measured. The constan was injected into nude mice with xenografted human bladder tumor. The biodistribution of the labeled ch BDI was studied with radioimmunoimaging. Results ch BDI showed desirable immunoreactive fraction (76%) and association constant (3 56×10 9M -1 ) in vitro and a terrific specific targeting effect in vivo. Conclusion ch BDI has fairly good function against human bladder tumor both in vitro and in vivo, and is promising in clinical use.