Ziehl-Neelsen-维多利亚蓝-碘绿复合染色法显示抗酸杆菌

Ziehl-Neelsen-Victoria blue-iodine green compound staining method showing leprotic bacillus

目的 :探讨显示皮肤活检组织中抗酸麻风杆菌、弹力纤维和细胞等组织成分的复合染色法。方法 :运用Ziehl Neelsen(Z N)苯酚复红染色液、维多利亚蓝 (Victoriablue ,VB)染色液、碘绿 (iodinegreen ,IG)和马汀氏黄 (Mar tiusyellow ,MY)染色液对皮肤活检组织进行复合染色。结果 :组织内结核样结节中麻风杆菌显示为红色 ,细胞核呈淡绿色 ,细胞浆无色 ,结节周围的弹性纤维呈蓝色 ,基质呈淡黄色。结论 :Z N原法染色单一 ,对比效果差 ,应用碘绿、马汀氏黄和维多利亚蓝复合的改造染色液 ,能更好显示组织中的麻风杆菌 ,以及包绕于结核样结节周围的弹力纤维等成分 ,该方法是一种对比清晰 。

Objective: To compare compound staining methods showing leprotic bacillus, elastic fibre and cell nucleus etc . in skin biopsy tissue. Methods: A compound staining dye composed of Ziehl Neelsen Phenol Fuchsin, Victoria blue, iodine green and Martius yellow were used in paraffin embeding skin biopsy tissue. Results: Leprotic bacilli showed red; cell nuclei, light green; cell cytoplasm, colorless; elastic fibres around tuberculoid nodules, blue; and essential substances, light yellow in skin biopsy tissue. Conclusion: Because the color was single in Ziehl Neelsen staining method, its contrast was poor.The ZN VB IG compound staining method may show leprotic bacilli, elastic fibres and cell nuclei etc. distinctly. Therefore, this is a reliable and nice color contrast compound staining method.