采用冷冻精子与新鲜精子进行卵胞浆内单精子注射的结果比较

A comparative study on the outcomes of intracytoplasmic sperm injection with frozen-thawed and fresh sperm

目的 比较冷冻精子与新鲜精子进行卵胞浆内单精子注射 ( ICSI)助孕技术的治疗效果。 方法 对 1 6 1对不育夫妇进行 1 6 3个辅助生殖技术治疗周期 ,其中采用冷冻精子 47个周期 ,比较了冷冻精子组与新鲜精子组 1 1 6个周期的受精率、卵裂率、A级胚胎率与临床妊娠率。 结果 冷冻精子组 (组 I)的受精率为 77.6 %、卵裂率为 92 .9%、A级胚胎率为 6 5.4%、临床妊娠率为 45.5% ;新鲜精液严重异常组 (组 II)分别为 54 .4%、94.0 %、45.7%及 2 5.0 % ;新鲜精液轻、中度异常组 (组 III)分别为 73 .5%、92 .5%、46 .8%及 2 9.3 %。组 I的受精率、A级胚胎率和临床妊娠率明显高于组 II( P<0 .0 5) ;与组 III比较无显著性差异 ( P>0 .0 5)。 结论 患者本身的精子质量直接影响 ICSI的受精率 ,精子冷冻复苏处理不影响 ICSI的受精率。

Objective: The aim of this study was to assess and compare the efficacy of intracytoplasmic sperm injection (ICSI) with frozen-thawed sperm and fresh sperm. Methods: 161 couples undergoing therapy of artificial reproduction technology were studied. The frozen-thawed sperm was used in 47 cycles. The fresh sperm was used in 116 cycles. The rates of insemination, embryo cleavage, A-grade embryo and clinic pregnancy were compared among them. Results: There were three groups: frozen-thawed sperms (group I), fresh sperms with severe abnormal semen (group II) or with mild-moderated abnormal semen (group III). The insemination rates were 77.6%, 54.4% and 73.5%, embryo cleavage rates were 92.9%,94.0% and 92.5%, A-grade embryo rates were 65.4%, 45.7% and 46.8% and clinic pregnancy rates were 45.5%,25.0% and 29.3%, respectively. Differences of the above-analyzed results between group I and II were significant (P<0.05) in insemination, A-grade embryo and clinic pregnancy. However, rates of insemination were not significantly (P>0.05) between group I and III. Conclusions: The results indicate that sperm quality of sterile patients throws the direct influence on the rate of insemination, but frozen-thawed sperms do not affect rates of insemination, embryo cleavage and A-grade embryo.