甲壳素纤维织品体外抗细菌和癣菌活性的研究

Studies of activity against bacteria and dermatophytes of textiles containing different concentrations of chitin in vitro

目的 观察甲壳素含量不同的针织布体外抑制或杀灭细菌或癣菌的作用。方法 采用不同细菌或癣菌接种量 (1× 10 6～ 1× 10 2 /mL) ,用体外抑菌试验和杀菌试验检测 10 0 %甲壳素纤维无纺布、2 0 %甲壳素针织布和 10 %甲壳素针织布对金黄色葡萄球菌、大肠杆菌、红色毛癣菌、紫色毛癣菌和石膏状毛癣菌的抗菌作用 ,实验中以普通全棉布作为对照。结果 作为对照的普通全棉布对上述细菌或癣菌无任何抑制作用。与普通全棉布比较 ,10 0 %甲壳素纤维无纺布、2 0 %甲壳素针织布和 10 %甲壳素针织布对金黄色葡萄球菌、大肠杆菌、红色毛癣菌、紫色毛癣菌和石膏状毛癣菌的抑制率 [抑制率 =(每毫升CFU值 每毫升接种量 ) /每毫升CFU值× % ]分别高于普通全棉布的 99.8%～ 99.9%、85 .2 %～ 89.6%和 74.2 %～ 85 .8% ,表明随甲壳素纤维含量逐渐增高 ,抑菌作用逐渐增强 (χ2 ≥ 18.2 5 ,P <0 .0 1)。所有甲壳素纤维织品均未发现有体外杀菌活性。结论 受试的甲壳素纤维织品无体外杀菌活性 ,但均有一定的体外抑制细菌或癣菌作用 ,以 10 0 %甲壳素纤维无纺布抑制作用最强 ,2 0 %甲壳素针织布次之 ,10 %甲壳素针织布较弱。

OBJECTIVE To observe the effects of textiles with different concentrations of chitin to inhibit or kill bacteria and dermatophytes in vitro . METHOD The assays for inhibiting or killing bacteria and dermatophytes under the different bacterial inoculation dosages in media (1×10 6～1×10 2/mL) in vitro were applied to determine the effects against S.aureus, E.coli, T.rubrum, T.violaceum and T.mentagrophyte of the textiles containing 100%, 20% and 10% of chitin respectively. Common whole cotton textile was used as a negative control in this study. RESULTS The common whole cotton textile did not show any the activities against the tested bacteria and dermatophytes. In comparison with common whole cotton textile, the inhibition percentages (inhibition percentage= CFU value per milliliter inoculation dosage per milliliter/CFU value per milliliter×%)against S.aureus, E.coli, T.rubrum, T.violaceum and T.mentagrophyte of textiles containing 100%, 20% and 10% of chitin were 99.8%～99.9%?85.2%～89.6% and 74.2%～85.8%, respectively. This result indicated that the effects of inhibiting bacteria or dermatophytes of the chitin contented textiles were increased corresponding to the chitin concentrations (χ 2≥18.25, P <0.01)?No killing bacterium or dermatophyte activities of the tested textiles could be found.CONCLUSION All the tested chitin contented textiles have no the activities of killing bacteria and dermatophytes in vitro. According to the results of the assays inhibiting bacteria and dermatophytes in vitro, the textile with the strongest inhibiting activity was that containing 100% of chitin, and then the textile containing 20% of chitin, and the textile containing 10% of chitin was the lowest.