小鼠牙本质涎磷蛋白(DSPP)基因敲除载体的构建

Construction of Mouse Dentin Sialophosphoprotein(DSPP) Gene Targeting Vector.

目的 :构建用于小鼠牙本质涎磷蛋白基因敲除的载体 ;方法 :PCR获得上、下游两条同源臂 ,将同源臂和正、负双向筛选标志按一定顺序克隆到载体 pBluescript中 ,序列测定确认正确后 ,NotI酶切使载体线性化 ;结果 :载体中各部分的排列顺序与预期一致 ;结论 :获得了小鼠牙本质涎磷蛋白基因敲除的载体 ,为以后的工作打下了基础。

Objective:To construct mouse DSPP gene targeting vector.Methods:To obtain 2 homology arms by PCR, and clone them, together with the positive and negative screening marker, into pBluescript in a definited order.Results:The sequences and order were consistent with expected.Conclsions:The DSPP gene targeting vector was constructed successfully, which made basis for further studies.