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咪唑克生对胰岛细胞的损伤效应及其机制

The effect of idazoxan on pancreatic β-cells and its mechanism
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摘要 目的　观察咪唑克生 (idazoxan)对克隆胰岛素分泌细胞 (BRIN BD11细胞 )的损伤效应。方法　采用MTS还原法测定胰岛细胞活力 ,用流式细胞计 (FACS)进行细胞周期分析来测定咪唑克生对BRIN BD11细胞DNA完整性的影响。结果　①咪唑克生明显降低BRIN BD11细胞的活力 ,破坏DNA ,且具有时间和剂量依赖性。②genistein、5 HT1A受体拮抗剂NAN190和蛋白激酶G (PKG)抑制剂———KT5 82 3对咪唑克生引起的BRIN BD11细胞损伤没有保护作用。结论　咪唑克生对克隆胰岛素分泌细胞具有细胞毒效应 。 AIM To investigate the effects of idazoxan on the viability and DNA integrity of clonal BRIN-BD11 cells. METHODS MTS reduction was used to check the viability of BRIN-BD11 cells. FACS cell cycle analysis was employed to determine the effects of idazoxan on DNA integrity of BRIN-BD11 cells. RESULTS ①Idazoxan markedly reduced the viability of BRIN-BD11 cells and resulted in DNA damage in time-and dose-dependent manner.②Genistein, 5-HT 1A antagonist NAN190 and PKG inhibitor-KT5823 had no protective effects against idazoxan-induced damage of BRIN-BD11 cells. CONCLUSION Idazoxan has cytotoxic effect and induces apoptosis of clonal pancreatic β-cells.
作者 高洪伟
机构地区 北京大学第三医院内分泌科
出处 《中国药理学通报》 CAS CSCD 北大核心 2003年第2期174-176,共3页 Chinese Pharmacological Bulletin
关键词 胰岛细胞 损伤效应 咪唑克生 BRIN-BD11 CELLS 细胞凋亡 idazoxan, BRIN-BD11 cells, apoptosis
分类号 R972 [医药卫生—药品]
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参考文献9

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中国药理学通报
2003年 第2期

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