摘要
目的 观察P物质 (SP)对人脐静脉张力的调节及其对体外培养人脐静脉内皮细胞 (HUVEC)膜上Ca2 + 通道及胞浆内游离Ca2 + 浓度 [(Ca2 + )i]的调控作用。 方法 应用常规生理记录仪 ,记录SP对人脐静脉张力的影响 ,用共聚焦激光扫描显微术和膜片钳单通道记录技术对体外培养HUVEC胞浆内 (Ca2 + )i、膜上Ca2 + 通道开放情况进行观察。 结果 SP具有内皮依赖性舒张人脐静脉的作用 ,促使体外培养HUVEC胞膜上Ca2 + 通道开放和胞浆内 (Ca2 + )i明显升高。 结论 SP对人脐静脉张力的舒张作用可能通过HUVEC胞浆内储存的 (Ca2 + )i释放和膜上Ca2 + 通道开放 。
Objective To observe the regulatory mechanism of substance P on the tension of human umbilical vein and the calcium ion of endothelial cells. Methods The tension was recorded by conventional physiological recording methods,and confocal laser scanning microscopy and patch\|clamp technique were used to observe the concentration of intracytosolic free calcium ion and the opening probability of membrane calcium ion channel of cultured human umbilical vein endothelial cells. Results Substance P induced the endothelial\|dependent relaxing of human umbilical vein and the increasing of the concentration of intracytosolic free calcium ion and of the opening probability of calcium ion channel in the cultured human umbilical vein endothelial cells.Conclusion\ Substance P could be function as the activator of the releasing of the intracellular stored calcium ion,and the inflowing of calcium ion from the outside cell bodies to relax human umbilical vein.
出处
《解剖学报》
CAS
CSCD
北大核心
2003年第1期36-39,共4页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目 (3 9670 3 82 )
关键词
钙离子
内皮细胞
膜片钳技术
P物质
共聚焦激光扫描显微术
人脐静脉
Calcium ion
Endothelial cell
Patch\|clamp technique
Substance P
Confocal laser scanning microscopy
Human umbilical vein