怀孕可增加小鼠海马齿状回的细胞增殖和神经发生

PREGNANCY INCREASES CELL PROLIFERATION AND NEUROGENESIS IN THE MOUSE DENTATE GYRUS

目的 观察怀孕对小鼠海马齿状回 (DG)和脑室下层 (SVZ)的细胞增殖和神经发生的影响。 方法 5 溴 2 脱氧尿苷 (BrdU)标记新生细胞结合免疫组织化学方法对脑切片进行BrdU、TuJ1及GFAP单标或双标染色。结果 怀孕小鼠齿状回的细胞增殖和神经发生明显高于正常未孕成年雌性小鼠 ,脑室下层中细胞增殖的数目在两者却无明显差异。在齿状回新生的细胞中大约有 80 %为新生的神经元 ,3%～ 5 %为神经胶质细胞。 结论 成年小鼠海马齿状回的细胞增殖和神经发生可能因怀孕而显著增多 ,这些新生的细胞对海马的功能可能有重要的影响。

Objective To observe cell proliferation and neurogenesis in the pregnant mouse dentate gyrus (DG) and the subventricular zone(SVZ). Methods Injection of the thymidine analog 5 bromo 2 deoxyuridine (BrdU)to determine the extent of cell proliferation combined with single or double immunohistochemistry staining with antibodies BrdU,TuJ1(class Ⅲ β tubulin,neuron specific early differentiation marker)and GFAP. Results The number of BrdU positive cells in the pregnant mouse dentate gyrus was significantly more than that of unpregnant mouse dendate gyrus but not in the subventricular zone.In dentate gyrus,approximately 80% of these cells were neuronal characteristics (TuJ1 immunoreactive)and 3%～5% of these cells were astrocytic characteristics(GFAP immunoreactive).Conclusion\ These findings suggest that pregnancy significantly increases cell proliferation and neurogenesis in the adult mouse dentate gyrus and present the possibility that these new cells exert an important influence on hippocampal function