螺旋藻多糖对HeLa细胞生长的影响

Effects of polysaccharide from spirulina platensis on the growth of HeLa cells

目的 :研究螺旋藻多糖 (polysaccharidefromSpirulinaplatensis,PSP)对体外培养的HeLa细胞生长的影响。方法 :MTT法测定螺旋藻多糖的抗肿瘤活性 ;光镜观察螺旋藻多糖对HeLa细胞形态学的影响 ;流式细胞术检测螺旋藻多糖对肿瘤细胞周期的影响。结果 :随螺旋藻多糖浓度的增加 ,HeLa细胞存活率逐渐降低 ,抑制率逐渐增加 ;光镜下的观察显示 ,螺旋藻多糖作用 2 4～ 4 8h后 ,细胞出现明显的形态学改变 ;流式细胞术证实螺旋藻多糖对肿瘤细胞存在周期特异性 ,使细胞发生G1期阻滞。结论 :螺旋藻多糖抑制HeLa细胞的增殖 。

Objective To investigate the effects of polysaccharide from Spirulina platensis (PSP) on the growth of HeLa cells. Methods The antiproliferation activities of PSP on HeLa cells were evaluated by MTT assay. The morphologic changes of HeLa cells were observed under light microscope. Cells division cycle was measured by flow cytometry. Results It was showed that with the increase of PSP concentration, the inhibited rate on HeLa cells increased and the survived rate declined; The HeLa cells exposed to PSP for 24～48h displayed significant morphologic changes; PSP had a specific effect on the HeLa cell division cycle, increased the number of cells in phase G 1. Conclusion These results indicated that the effects of PSP on HeLa cells may probably be attributed to increase of the number of cells in phase G 1.