灵芝多糖肽的抗氧化作用

Antioxidant effect of Ganoderma polysaccharide peptide

目的 研究灵芝多糖肽 (GLPP)的抗氧化作用及其机制。方法 Cu2 + 诱导人血清低密度脂蛋白 (LDL)氧化及iv四氧嘧啶 ( 75mg·kg- 1 )引发小鼠氧自由基损伤。结果 GLPP使LDL氧化修饰减少 ,氧化产物REM降低。GLPP5 0 ,10 0 ,2 0 0和 40 0mg·kg- 1 ip 2 0d ,使血清和心肌匀浆的丙二醛 (MDA)水平下降 ,谷胱甘肽过氧化物酶 (GSHpx)升高 ,超氧化物歧化酶 (SOD)减少 ,过氧化氢酶 (CAT)不变。结论 GLPP具有体内外抗氧化作用 。

Aim To study the antioxidant effect of Ganoderma polysaccharide peptide (GLPP) and its mechanism. Methods Copper was used as oxidant to induce low lipoprotein (LDL) oxidative modification, and alloxan was given iv to induce reactive oxygen species (ROS) injury in mice. Results GLPP decreased oxidation of LDL and the relative electrophoretic mobility (REM) of oxidative product of LDL. After GLPP was given ip for 20 days, the concentration of malondialdehyde(MDA) in serum and heart of mice was decreased. The GSHpx enzyme activity was increased, while the SOD level was decreased. The catalase(CAT) levels were not significantly changed by GLPP. Conclusion GLPP showed antioxidant effect by scavenging ROS or enhancing the enzyme activity of GSHpx in vivo and in vitro .