皮肤切创组织IL-2、TNF-α时间相关性表达的ELISA分析

A analysis of ELISA on the time-related expression of IL-2 and TNF-a during the healing process of wound in rat skin

目的了解细胞因子在创伤及修复过程中的表达的含量变化与损伤时间的关系。方法用免疫酶联吸附方法(ELISA)检测不同损伤时间(生前伤0.5～168h)实验动物皮肤切创组织中IL-2和TNF-a的表达水平。结果IL-2、TNF-a在造创后0.5h即升高,并分别在造创后3h和1h到达峰值,在造创后48h均有反弹,在72h和168h持续升高。结论上述细胞因子在创口修复中的蛋白表达水平呈时间相关性特点。

Objective In order to explore the relationship between the expression change of cytokines and the wound age during the healing process of rats skin wound. Methods A assay of ELISA were performed on intravital skin wounds(after incision 0.5-168 h) to detect the dynamics expression level of IL-2, TNF-a. Results The level of IL-2 and TNF-a increased at 0.5 h after wounding, then got to a peak at 3 h and 1 h after injure respectively. Rebound of TNF and IL-2 levels were shown at 48 h after wounding, and both cytokine levels were inclined to elevating between 72h and 168h after wounding. Conclusion The cytokine level changes suggest they have a time-related expression during wounds healing process.