大鼠皮肤钝器伤血管内皮生长因子表达变化的研究

A study on the expression of VEGF in the blunt injury of rat's skin

目的观察大鼠皮肤钝器伤后VEGF表达的变化规律。方法应用免疫组化技术观察大鼠皮肤钝器伤后不同时间(1h、3h、6h、12h、1d、3d、5d、7d、9d)VEGF的表达情况,并用MIAS图像分析系统进行图象分析。 结果正常对照组大鼠皮肤有低水平VEGF表达,钝器伤后VEGF表达逐渐增加,于伤后7d达峰值,伤后9d开始下降。 结论皮肤钝器伤可诱导VEGF表达增加,其时序性变化可望作为一种客观指标用于法医学损伤时间的推断。

Objective To evaluate changes of VEGF in skin after blunt injury. Methods The rats of injury groups were subjected to skin blunt injury by free-falling iron hammer. The samples taken at 1h, 3h, 6h, 12h,1d, 3d, 5d, 7d, 9d after injury were studied by immunohistochemistry and MIAS image analysis system. Results In the skin of normal control group the expression level of VEGF was low. The increase of VEGF could be observed at 1 day after injury, reached peak at 7 days and declined at 9 days. Conclusion Blunt injury in the skin could induce the expression of the VEGF. Moreover, the change pattern of VEGF level was quite regular with time and could be used to estimate the time after skin injury.