化学点燃癫痫大鼠在水迷宫中学习记忆能力的测定

The assessment of the abillity of the learning and memory in picrotoxin-kindled rats after the Morris water maze task training

目的 观察印防己毒 (Picrotoxin ,PTX)化学点燃癫痫大鼠在水迷宫中学习记忆能力与发作频率及类型的关系 ,为进一步研究癫痫患者记忆损害的治疗提供线索。方法 34只雄性SD大鼠随机分为点燃组和对照组 ,分别用PTX和生理盐水腹腔注射 ,根据点燃情况点燃组再分为全面发作 (A)、频繁发作 (B)和部分发作 (C)组 ,对照组即为迷宫训练 (D)组 ,然后进行水迷宫行为测试 ,评价其学习记忆能力。结果 癫痫大鼠在水迷宫测定中 ,除B组第 1天的成绩较对照组差外 ,其余各组及B组在第 2、3、4、5天中寻找平台的潜伏期时间与对照组相比没有显著性差异。点燃各组对平台空间位置的记忆能力较对照组要差 ,差异有显著性。结论 首次用化学点燃模型研究癫痫大鼠在水迷宫中的学习记忆能力后发现 ,PTX化学点燃癫痫大鼠在水迷宫中学习记忆能力下降 ,发作频繁者学习记忆受损明显 ,但与发作的严重程度无关。

Objective To study the ability of the learning and memory in picrotoxin kindled rats after the Morris water maze task training and its' relationship with the seizure type and frequency.Methods 34 male SD rats were randomly divided into kindle group and control group(group D, maze group). Picrotoxin and saline were injected separately through intraperitoneal, according to the state of the seizure, kindle group were then divided into group A(general seizure group), group B(frequent seizure group) and group C(partial seizure group). Then the learning and memory abilities were tested after Morris water maze training.Results during the first day of place navigation, rats in group B performed worse than the control group( P < 0.05 ), except for this, there is no difference between all kindle groups(including group B during the other 4 days) and control group in the latency of escape. In the spatial probe test, the times across the original platform of the kindled rats were fewer than the control group. Conclusion The ability of the learning and memory in picrotoxin kindled rats was assessed after the Morris water maze task training for the first time and found that the learning and memory of picrotoxin induced epileptic rats were impaired. They are associated with seizure frequency, but in no relation with seizure type.