儿茶素类化合物对四氯化碳致大鼠慢性肝损伤的保护作用

Protective Effect of Catechins against CCl_4-Induced Chronic Hepatic Injury in Rat

目的 观察儿茶素类化合物保肝降酶的作用。方法 制备大鼠的四氯化碳 (CCl4)慢性肝损伤模型 ,观察儿茶素类化合物对肝损伤大鼠血清丙氨酸转氨酶 (ALT)、天冬氨酸转氨酶 (AST)、丙二醛 (MDA)等的影响 ,以及肝组织羟脯氨酸含量和肝脏组织结构的变化。结果 儿茶素类化合物给药 2个月后 ,中、高剂量给药组 (10 0 ,2 0 0mg/kg)均能降低血清ALT的活力以及脂质过氧化产物MDA和肝组织羟脯氨酸的含量 (P <0 0 1或P <0 0 5 ) ,其降低肝组织羟脯氨酸含量的作用与联苯双酯相当。肝组织的病理改善情况两药相似 ,而抗膜脂质过氧化作用优于联苯双酯。结论 儿茶素类化合物具有保护化学性肝损伤作用 ,有降低转氨酶活力。

Objective To examine roles of catechins in protecting liver and decreasing enzyme activity.Methods Chronic CCl 4 induced hepatic injury model was prepared in rat.Effects of catechins on levels of ALT,AST,MDA in rat serum and hydroxyproline levels in liver tissue as well as hepatic morphologic changes were observed.Results After treated with catechins for two months,ALT activities,MDA levels in serum and hydroxyproline levels in liver tissue were significantly decreased in the two catechin groups(100, 200 mg/kg respectively,P<0 01,or P<0 05).Catechin was similar to biphenyl dimethylesterate in decreasing hydroxy proline levels and improving pathological changes in liver tissues,however,better than biphenyl dimethylesterate in anti peroxidization of membrane lipid.Conclusions Catechins had the protective effect against chronic chemical hepatic injury by decreasing transaminase activities and alleviating hepatic pathologic injury.