摘要
β-Nerve growth factor (β NGF) mRNA levels in hippocampa from C57BL/6J mice of different ages were compared by semi-quantitative RT-PCR method, taking β-actin gene as an internal control. The levels of 6-and 12-month-old C57 mice were much lower compared with that of the l-month-old mouse. However, there was no significant difference between these two groups. This result suggests that gene transcription level of hippocampus β NGF in a C57, mouse descends evidently as it grows up. Base T at Site 294 of β NGF cDNA was substituted by C in the C57, mouse, as revealed by DNA sequencing for the first time. Nevertheless, this polymorphism of nucleotide did not make any difference in amino acid composition.
采用半定量 ＲＴ－ＰＣＲ法测定了１，６，１２月龄 Ｃ５７小鼠海马βＮＧＦ ｍＲＮＡ水平，将β肌动蛋白基因作为内标。结果表明， ６， １２月龄两组间无显著性差异，但均显著低于１月龄组，说明Ｃ５７小鼠发育过程中其海马 βＮＧＦ基因转录水平明显降低。另外，发现Ｃ５７小鼠海马 β ＮＧＦ基因第２９４位碱基为Ｃ，与普通小鼠的Ｔ不同，但这种碱基差异并不影响氨基酸组成。
