星形细胞起源肿瘤的恶性进展与Topo-Ⅱα表达关系的研究

Study on the relationship between topoisomerase-Ⅱ alpha expression and malignant progression of astrocytic tumors

目的研究拓扑异构酶-Ⅱα(topoisomerase-Ⅱα,Topo-Ⅱα)在星形细胞起源肿瘤中的表达与病理分级的关系,并探讨Topo-Ⅱα对判断此类肿瘤患者预后的价值。方法应用免疫组织化学二步法对49例不同级别的星形细胞起源肿瘤标本Topo-Ⅱα蛋白表达进行分析,以评价阳性细胞百分数与星形细胞起源肿瘤恶性程度的关系。结果随着肿瘤病理级别的提高,细胞异型性明显增加、核染色增强、对比明显;而且Topo-Ⅱα标记指数亦随之升高,低级别(Ⅰ、Ⅱ级)与高级别(Ⅲ、Ⅳ级)肿瘤之间的差异具有显著性意义(P<0.01),但Ⅲ、Ⅳ级肿瘤之间的差异则无显著性意义(P>0.05)。结论Topo-Ⅱα可较为准确地反映星形细胞起源肿瘤的增殖潜能和恶性程度,对判断脑胶质瘤患者的预后具有重要参考价值。

Objective To study the relationship between topoisomerase-Ⅱ alpha (Topo-Ⅱ α) expression and pathological grades of astrocytic tumors, and approach the value of Topo-Ⅱ α in evaluation the prognosis of astrocytic tumors. Methods The Topo-Ⅱ α expression in 49 cases of astrocytic tumors with various pathological grades was detected by two step immunohistochemical staining to evaluate the relationship between the percentage of positively stained cells and the malignant intensity. Results The Topo-II α labeled index was increased along with the increasing of pathological grades of the tumors and significant heterotypic character of cells, deepening of nuclear staining with more clearly contrast were also occurred. There was significant difference of this index between lower grades groups (Ⅰ, Ⅱ) and higher grades groups (Ⅰ, Ⅱ) of astrocytic tumors (P < 0.01), but the difference between grade Ⅲ and Ⅳ was not significant (P > 0.05). Conclusion Topo-Ⅱ α detection may accurately reflect the malignant intensity and potential cell proliferation activity of astrocytic tumors, and provide an important reference for estimating the prognosis of the patients with astrocytic tumors. But the problem of whether the Topo-Ⅱα expression is a guide parameter for setting up the therapeutic scheme in astrocytic tumors should be further studied.