珠穆根散改善血管性痴呆大鼠记忆障碍的实验研究

An experimental study of zhumugensan′s effects on memory disorders in rats with vascular dementia

目的 :研究珠穆根散对血管性痴呆大鼠记忆障碍的作用及其可能的作用机制。方法 :通过夹闭大鼠两侧颈总动脉 ,制作大鼠血管性痴呆模型。采用水迷宫和电迷宫 ,在喂药前和喂药结束后对大鼠进行行为检测以检验大鼠记忆能力的变化。喂药结束后 ,用高效液相色谱──荧光检测法测定各组大鼠海马组织去甲肾上腺素的含量变化。用电镜观察各组大鼠海马CA3区超微结构的改变。结果 :喂珠穆根散 5 0天后 ,药物治疗组与治疗对照组相比 ,大鼠通过迷宫的时间显著缩短 (P <0 0 5 ) ,说明其记忆力有明显的改善 ;大鼠海马去甲肾上腺素含量明显增高(P <0 0 5 )。治疗对照组大鼠海马CA3区超微结构病变以神经元细胞核和核膜变化最为显著 ,珠穆根散治疗组大鼠海马CA3区超微结构病变明显减轻。结论 :珠穆根散对血管性痴呆大鼠记忆障碍有明显的改善作用 ,可能是与其调节去甲肾上腺素的代谢 ,增强海马CA3区神经元对缺血损伤的抵抗性有关。

Objective:To study the effect of Zhumugensan(a kind of Tibetan compound recipe)on the memory disorders of the rat′s with vascular dementia(VD)and its probable mechanism.Method:The VD model was estabished by the ischemia-reperfusion which was made by repeated occlusion of the rat′s bilateral carotid arteries.In order to detect the rat′s memory ability,the rat′s bebavior was tested by the water maze and electric maze before and after feeding drugs.The high-performance liquid chromatography with fluorometric detection was carried out to observe the norepinephrine(NE) level changes in rat′s hippocampus after feeding drugs.The changes of ultrastructure the rat′s hippocampus CA3 subfield were observed under the electron microscope.Result:Comparsion of the Zhumugensan-treated group with the control group showed that rat′s hippocampus NE level increased obviously( P <0.05);the time which the rats passed through the maze reduced obviously( P <0.05).It indicated that the rat′s memory ability had improved remarkably.The ultrastructural alterations of the control group rat′s hippocampus CA3 subfield were the nucleus of the neuron and the nuclear membrane structure.The ultrastructural alterations of the Zhumugensan-treated group rat′s hippocampus CA3 subfield alleviated markedly.Conclusion:Zhumugensan could improve the memory disorders in VD rats.The mechanism might relate to its modulating the metabolism of NE and enhancing the resistance of the rat′s hippocampus CA3 subfield against the ischemic injury.