摘要
Carbon fiber nanoelectrodes(200-300 nm) were firstly used to amperometricaly monitor the dopamine release from single PC12 cells with temporal resolution and especially more higher spatial resolution than those obtained by using microelectrodes. When the nanoelectrode was in a distance 1 μm above the PC12 cell, only one peak signal corresponding to a single vesicle exocytotic event was detected caused from the stimulation with 1 mmol/L nicotine. The spatial difference of exocytosis was also detected by placing the electrode onto the different locations of the cell body, the results have demostrated that the spatial distribution of dopamine in cells is not uniform and the time for stimulating secretion is very different. Nanoelectrodes electrochemical method can provide a powerful tool for observing the temporal and spatial characteristics of the secretion from single cells directly.
Carbon fiber nanoelectrodes(200-300 nm) were firstly used to amperometricaly monitor the dopamine release from single PC12 cells with temporal resolution and especially more higher spatial resolution than those obtained by using microelectrodes. When the nanoelectrode was in a distance 1 μm above the PC12 cell, only one peak signal corresponding to a single vesicle exocytotic event was detected caused from the stimulation with 1 mmol/L nicotine. The spatial difference of exocytosis was also detected by placing the electrode onto the different locations of the cell body, the results have demostrated that the spatial distribution of dopamine in cells is not uniform and the time for stimulating secretion is very different. Nanoelectrodes electrochemical method can provide a powerful tool for observing the temporal and spatial characteristics of the secretion from single cells directly.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2003年第3期425-427,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金 (批准号 :2 9975 0 2 0 )资助
