高效液相色谱法和蒸发光散射检测器分析大豆磷脂分子种

Analysis of Soybean Phospholipids Molecular Species by HPLC Using Evaporative Light Scattering Detector

用高效液相色谱法 (HPLC)在半制备硅胶柱上将大豆磷脂分离成各类磷脂 ,柱后收集磷脂酰乙醇胺(PE) ,磷脂酰胆碱 (PC) ,磷脂酰肌醇 (PI) ,以甲醇 氯仿 乙腈 水为流动相 ,用梯度洗脱程序在ProdigyODS(3 )柱上分离分子种 ,PE、PI和PC各被分离成 9个、10个和 11个分子种。组分定性由HPLC峰的脂肪酸组成确定。此方法溶剂易得 ,简单快速 (仅需 3 0min) ,可用于分离各种大豆磷脂分子种。

Soybean phospholipids were separated into phospholipids on a semi-preparative s ilica gel column using HPLC. Phosphatidylcholine(PC), phosphatidylethanolami ne(PE) and phosphatidylinositol(PI) fractions were collected. Their molecular sp ecies compositions were analysed on a revers phase Prodigy ODS(3) column and a g radient elution program performed with chloroform-methanol-acetonitrile-water as a flow phase. Phosphatidylcholine, phosphatidylethanolamine and phosphatidyl inositol were separated into 11, 9 and 10 molecular species respectively. The molecular species identities were assign ed from fatty acid compositions determined by gas chromatography(GC) . The method has advantages of simplicity and availability of solvents .