雌激素对氧化低密度脂蛋白诱导单核细胞趋化因子受体CXCR2的影响

Effects of 17 estradiol on chemokine receptor CXCR2 expression in monocytes incubated with oxidized low density lipoproteins

目的 研究 17β 雌二醇对氧化低密度脂蛋白 (oxLDL)诱导单核细胞合成趋化因子受体CXCR2的影响。 方法 单核细胞的研究模型为人前单核细胞系U937细胞。流式细胞仪测定单核细胞趋化因子受体CXCR2的蛋白表达 ,逆转录PCR测定其mRNA水平。 结果 oxLDL(5 0μg/ml)可明显促进U937细胞的CXCR2mRNA及蛋白表达 ;17β 雌二醇 (5 0nmol/L)预处理抑制了oxLDL(5 0 μg/ml)诱导的U937细胞CXCR2的mRNA及蛋白表达 ,且 17β 雌二醇 (5～ 5 0 0nmol/L)抑制oxLDL(5 0 μg/ml)诱导U937细胞合成的CXCR2蛋白呈正向量效关系。 结论 雌激素抑制oxLDL诱导U937细胞产生的趋化因子受体CXCR2表达。

Objective To study the effects of 17estradiol on chemokine receptor CXCR2 expression in monocytes incubated with oxidized low density lipoproteins (oxLDL). Methods Human monocytic U937 cells were used as a model of monocytes. Expressions of CXC chemokine receptor CXCR2 mRNA and protein were measured by RT-PCR and FACS, respectively. Results The oxLDL(50 μg/ml) significantly upregulated CXCR2 expression in U937 cells. 17estradiol (50 nmol/L) reduced expressions of CXCR2 mRNA and protein in U937 cells incubated with oxLDL (50 μg/ml) , and the decrease of the CXCR2 protein was dependent on the concentration of 17estradiol (5-500 nmol/L). Conclusions 17estradiol can obviously reverse oxLDL-induced CXC chemokine receptor CXCR2 expression in U937 cells.