胡黄连苷-Ⅱ在体外对PC12神经细胞损伤的保护作用

Protective effect of picroside-Ⅱ on the damage of cultured PC12 cells in vitro

目的 :考察胡黄连苷 Ⅱ对PC12神经细胞损伤的保护作用。方法 :采用细胞培养的方法 ,建立PC12神经细胞双氧水 (H2 O2 )损伤模型。通过观察细胞形态 ,测定细胞存活率 (MTT法 ) ,乳酸脱氢酶(LDH)漏出率 ,细胞内氧化活性物质 (ROS)水平 ,检测胡黄连苷 Ⅱ对PC12细胞损伤的保护作用。结果 :同造模组比较 ,胡黄连苷 Ⅱ 0 .5、5 .0mmol·L-1能减轻H2 O2 引起的对PC12神经细胞的损伤 ,明显提高细胞的存活率 ,减少LDH的释放量 ,降低细胞内ROS水平。结论 :胡黄连苷 Ⅱ对PC12神经细胞损伤具有明显的保护作用。

AIM: To investigate protective effect of picroside-Ⅱ on the damage of cultured PC12 cells. METHODS: The damage of cultured PC12 cells was made by hydrogen peroxide. The protection of picroside-Ⅱ was observed by morphological identification, colorimetric MTT assay, effluxes of LDH, and ROS level. RESULTS: 0.5 and 5 mmol·L -1 of picroside-Ⅱattenuated the damage of PC12 cells by increasing significantly the survival rate, decreasing the changes in cell morphology, and lowering LDH release from cells into culture medium and ROS level. CONCLUSION: Picroside-Ⅱ can obviously protect the cultured PC12 cells from damage induced by hydrogen peroxide.