白细胞介素-1受体拮抗剂拮抗豚鼠哮喘的相关机制

Effect of interleukin-1 receptor antagonist on the rat models with asthma1

目的 :研究白细胞介素 1受体拮抗剂 (IL 1ra)拮抗豚鼠哮喘的机制。方法 :建立致敏豚鼠模型 ,吸入不同浓度的IL 1ra ,引喘豚鼠 ,检测引喘后 8d血清、肺组织中 ,内皮素 1(ET)、一氧化氮 (NO)、以及血液、肺泡灌洗液 (BALF)中嗜酸性粒细胞 (EOS)的变化情况。结果 :IL 1ra可以明显升高血清和肺组织中的NO浓度 ,降低血清和肺组织中的ET含量 ,减少血液和肺泡灌洗液中的EOS的数量 ;高剂量组缓解作用较强。结论 :IL 1ra可增加机体内的NO浓度 ,降低ET的含量 ,减少EOS浸润。IL 1ra对豚鼠哮喘具有拮抗作用。

AIM: To study the protective effect of interleukin-1 receptor antagonist (IL-1ra) on guinea pig models with asthma. METHODS: Ovalbumin sensitized guinea pigs as asthma models were treated with IL-1ra for 8 days. At the end of the treatment, the concentration of endothelin (ET) and nitric oxide (NO) in the blood and in the lung tissue were determined, and the count of eosinophil (EOS) in the blood and the bronchoalveolar lavage fluid (BLAF) was measured as well. RESULTS: IL-1ra lowered ET content in the blood and lung tissue, and the count of EOS in the blood and BLAF as well. But it increased the activity of NO, and the high dose group showed a better effect. CONCLUSION: The inhalation of nebulized IL-1ra has an effect against asthma.