七种抗菌药物对创伤弧菌感染小鼠实验治疗的时效和量效关系

Dose and time-effect relationship of seven antimicrobial agents in treatment of vibrio vulnificus infection in mice

目的 :探讨 7种抗菌药物对创伤弧菌感染小鼠实验治疗的时效和量效关系。方法 :创伤弧菌(6.0× 10 11CFU·L-1)经ip感染小鼠后 1h ,分别应用ip亚胺培南 西司他丁 (泰能 )、氯霉素、多烯环素、硫酸奈替米星、头孢哌酮钠、哌拉西林钠、乳酸左旋氧氟沙星抗菌药物 5个剂量级进行治疗 ;小鼠经ip感染创伤弧菌后 0 .5、1、2、3h分别用人体治疗量的上述抗菌药物进行治疗 ,观察存活小鼠数及小鼠的脏器的超微结构变化。结果 :创伤弧菌感染后 1h应用 5个剂量级即使是小剂量这些抗菌药物对创伤弧菌感染的小鼠均有显著治疗作用 ,大剂量抗菌药物治疗后血培养阴性 ,脏器超微结构损伤恢复 ,感染0 .5～ 1h后应用人体剂量的这些抗菌药物治疗效果较好 ,2h后氯霉素、奈替米星、头孢哌酮钠、乳酸左旋氧氟沙星疗效最好 ,3h后治疗效果差 ,加大剂量抗菌效果增加 ;泰能治疗效果较差。结论 :及早大剂量应用这些抗菌药物对创伤弧菌感染小鼠有较好的治疗效果 ,为临床治疗该病时选用氯霉素、奈替米星、头孢哌酮钠、乳酸左旋氧氟沙星提供可靠依据。

AIM: To investigate the dose and time-effect relationship of 7 antimicrobial agents in experimental treatment of vibrio vulnificus infection in mice. METHODS: Vibrio vulnificus ( 6.0×10 11 CFU·L -1) was introduced intraperitoneally into the right abdominal cavity. After 1 h, 7 antimicrobial agents were given intraperitoneally using 5 dose levels respectively, and (were given intraperitoneally) using human therapeutic dose after 0.5, 1, 2, 3 h of infection, respectively. 7 antimicrobial agents were imipenem lcilastatin, chloramphenicol, doxycycline hydrochloride, netilmicin sulfate, cefoperazone sodium, piperacillin sodium, and levofloxacin lactate. The numbers of survival mice and the supermicrostructure change of organs were observed. RESULTS: With high dose of the seven agents, blood culture showed negative, and organ supermicrostructure injure recovered. 7 antimicrobial agents produced satisfactory therapeutic effect against experimental vibrio vulnificus infection using human therapeutic dose after 0.5～1 h of infection. Chloramphenicol, netilmicin sulfate, cefoperazone sodium and levofloxacin lactate had the better therapeutic effect after 2 h of infection, and weak effect after 3 h. Imipenem showed a weak effect. CONCLUSION: The treatments with these 7 antimicrobial agents early can obtain satisfactory therapeutic effect against experimental vibrio vulnificus infection in mice.