GC-MS建立石牌广藿香挥发油指纹图谱方法学研究

Study on GC-MS fingerprint analysis of volatile oil of Pogostemon cablin (Blanco) Benth. Cultivated in GAP Plots

目的 :建立石牌广藿香挥发油GC MS分析色谱条件 ,初步拟定石牌藿香挥发油特征指纹图谱指标成分群 ,阐明石牌藿香的道地特性 ;方法 :采用GC MS法对不同采集期石牌藿香 ,以及同基地栽培的高要、湛江藿香挥发油进行主成分比较分析 ;结果 :以 1 1个主要共有峰为评价指标 ,GC MS色谱条件的精密度、稳定性、重现性良好 ;固定产地的石牌藿香稳定性好 ,不同采收期主成分基本相同 ,其中广藿香酮的含量随种植时间的延长而明显增高 ;与同地栽培的高要、湛江藿香相比 ,广藿香酮、广藿香醇等含氧组分以及不含氧的倍半萜烯类主成分的相对含量均有明显差异 ;结论 :GC MS法分析广藿香挥发油主成分 ,方法准确可靠 ;其本身具有的多成分同时定性的优势 ,为阐明道地药材牌香与高要。

Objective: To establish the method of fingerprint analysis on volatile oil of Pogostemon cablin (Blanco) Benth. cultivated in Shipai country by GC MS, and to study the main characteristic components. Methods: The main components of the volatile oil of Pogostemon cablin (Blanco) Benth. from different colletion time in Shipai country were determined by GC MS, and compared with the volatile oil of “Gaoyao huoxiang” and “Hainan huoxiang” cultivated in the same GAP plots. Results: RSD of precision and reproducibility and stability is in the range of 5%, and the main components of the volatile oil of “Shipai huoxiang” from different collection time are nearly similar, and the content of Pogostone increases along with cultivation time, and the content of Pogostone and Patchoulic alcohol are significantly differences compared with the “Gaoyao huoxiang” and “Hainan huoxiang”. Conclusion: The method is reliable, accurate and can be used for quality control of volatile oil of Pogostemon cablin (Blanco) Benth.