基质金属蛋白酶-9与原发性肝癌侵袭转移关系的初步研究

Significance of Matrix Metalloproteinase mRNA Expression in Invasion and Metastasis of Hepatocellular Carcinoma

目的 探讨原发性肝癌中MMP -9基因的表达 ,研究其与原发性肝癌侵袭转移的关系。方法 采用逆转录 -聚合酶链式反应技术 (RT -PCR ) ,对 41例原发性肝癌手术切除肿瘤组织、癌旁组织标本的MMP -9基因进行检测 ,并结合随访资料进行分析。结果 肝癌组织和癌旁 1cm、5cm组织中分别有 70 .7%、48.9%、41.5 %MMP -9表达阳性 ,癌组织MMP -9表达水平显著高于癌旁 5cm组织 (P <0 .0 1) ,癌组织与癌旁 1cm组织比较 ,MMP -9表达水平无显著性差异。癌组织MMP -9表达水平与肿瘤复发、有无包膜、门静脉癌栓显著相关 (P <0 .0 5 )。结论 MMP -9基因的过度表达与肝癌侵袭转移密切相关 。

Objective To investigate the role of MMP 9 in the invasion and metastasis of the hepatocellular cancer.Methods RNA was extracted in 41 surgical specimens of HCC and reverse transcript polymerase chain reaction(RT PCR) was carried out for detection of MMP 9 expression.Results MMP 9 was expressed positively in 70.7% of hepatocellular carcinoma tissues,48.9% of paracacinoma 1 cm and 41.5% of paracarcinoma 5 cm tissues.The expression level of MMP 9 in tumor tissues was obviously higher than that in the tissues of paracacinoma 5 cm( P <0.01),but was not higher than that in the tissues of paracacinorma 1 cm.The expression of MMP 9 were significantly correlated to recurrence and pv emboli and poor encapsulated( P <0.05).Conclusion The expression of MMP 9 correlates closely to the recurrence or metastasis of HCC.High expression levels of MMP 9 may be as a predictor for poor prognosis of HCC.