结核性脑膜炎患者脑脊液中肿瘤坏死因子α、磷脂酶A_2、前列腺素E_2的改变及意义

The Clinical Significance of Changes of levels of Tumour Necrosis-α, Prostaglandin E_2, and Phospholipase A_2 in Cerebrospinal Fluid of Patients with Tuberculous Meningitis

目的 :探讨肿瘤坏死因子 α(TNF α) ,磷脂酶A2 (PLA2 ) ,前列腺素E2 (PGE2 )在结核性脑膜炎患者脑脊液中的变化及临床意义。方法 :TNF α测定采用ELISA法 ;PGE2 采用放射免疫法 ;PLA2 测定采用 [3 H] 油酸标记的大肠杆菌膜磷脂为底物的放射化学法。结果 :结核性脑膜炎患者脑脊液中TNF α、PLA2 及PGE2 均较对照组显著升高 ;经抗结核药物加激素联合治疗后TNF α、PLA2 及PGE2 均显著下降 ;相关分析显示TNF α与PLA2 呈正相关 ,而PLA2 与PGE2 无显著相关关系。结论 :TNF α、PLA2 及PGE2

Objective: To investigate the changes of levels of tumour necrosis α(TNF α), prostaglandin E 2 (PGE 2 ), and phospholipase A 2(PLA 2)in cerebrospinal fluid of patients with tuberculous meningitis and their clinical significances.Methods: The TNF α,PGE 2,and PLA 2 in CSF were determined by ELISA,radioimmmunoassay,and radiochemical assay,respectively.Results:The levels of TNF α,PGE 2,and PLA 2 in CSF of the patients with tuberculous meningitis elevated significantly compared with that in the control group. After treatment with anti tuberculous drugs and glucocorticoids such as dexamethasone, the levels of the TNF α,PGE 2, and PLA 2 in CSF significantly decreased.Correlation analysis showed that there was a positive correlation between the levels of the TNF αand the PLA 2, but no correlation was found between the levels of the PGE 2 and the PLA 2.Conclusion:TNF α, PLA 2,and PGE 2 may participate in the pathogenesis of the tuberculous meningitis and play an important role in this condition.