摘要
目的 通过研究 6个STR基因座FGA ,TPOX ,D3S135 8,vWA ,D8S1179,D2 1S11的遗传多态性 ,了解它们在河南汉族人群中的多态分布 ,与其他群体进行比较 ,得出遗传距离 ,并了解它在法医学中的应用价值。 方法 采用多聚酶链式反应扩增这 6个基因座 ,采用非变性聚丙烯酰氨凝胶电泳银染显色分析。 结果 得出这 6个基因座在河南汉族人群中的基因频率 ,并计算得出杂合度、个体识别率、非父排除率 ,与其他群体比较得出进化距离。 结论 这 6个基因座有较高的杂合度 ,并且具有相对遗传稳定性 ,在人群中的分布符合Hardy -Weinberge平衡 ,有较高的法医学价值 ,可以应用于个体识别和亲权鉴定。
Objective To investigate the alleles frequencies of six STR loci: FGA,TPOX,D3S1358,vWA,D8S1179,D21S11 in Han population in Henan province. Methods DNA was extracted with phenol-chloroform from EDTA-blood samples of the unrelated individuals and families in Henan Province and amplified with PCR technique. The PCR product was analyzed with the undenatured PAGE vertical electrophoresis and silver-stain. Results The freqencies of the six STR loci were obtained, compared the data from six populations. The frequencies are in the good agreement with Hardy-Weinberg equilibrium. The heterozygosity, discrimination power and cumulation chance of exclusion in six STR loci were calculated. Conclusion The six STR loci in this study have higher heterozygosity and relative genetic stability. It is suggested that these STR loci can be used in individual identification and paternity test in forensic medicine.
出处
《法律与医学杂志》
2003年第1期37-40,共4页
Journal of Law & Medicine
关键词
遗传多态性
短串联重复
聚合酶链式反应
Genetic polymorphism, Short tandem repeats, Polymerase chain reaction