骨骼肌条件培养液对肺癌细胞生长抑制作用的实验研究

An Experimental Research on Inhibitory Action of Skeletal Muscle Conditioned Medium on Metastases of Lung Carcinomas

背景与目的:恶性肿瘤可转移至机体所有器官,但在骨骼肌中罕见。本实验拟通过研究骨骼肌微环境因素对不同转移潜能肺癌细胞增殖的影响,探讨骨骼肌内转移瘤罕见这一现象的发生机制。方法:原代培养新生Wistar大鼠骨骼肌细胞,用MTT法分析和比较骨胳肌细胞条件性上清液(murineskeletalmuscleconditionedmedium,MMCM)对不同转移潜能肺癌细胞的体外抑瘤作用,以阿霉素作为MMCM的阳性对照,以金黄地鼠肾细胞株BHK-21作为肺癌细胞的阴性对照。结果:不同转移潜能肺癌细胞,即人肺巨细胞癌低转移株PLA-801C和人肺巨细胞癌高转移株PLA-801D与MMCM共同培养后,PLA-801C细胞在MMCM稀释至50%时其生长增殖即不受明显影响,而PLA-801D细胞的生长在MMCM稀释至原液的6.25%时仍受显著抑制。而MMCM在各稀释浓度点对BHK-21细胞的生长均无显著抑制作用。结论:新生大鼠骨骼肌细胞上清液可选择性抑制恶性肿瘤细胞增殖;MMCM对人肺巨细胞癌高转移株较低转移株细胞的生长抑制作用更明显。

BACKGROUND &OBJECTIVE:Malignant tumors spread and metastasize in the majority of the organs, but are very rare in skeletal muscles. This study was conducted to explore the effect of organic microenvironment of skeletal muscles on the proliferation of pulmonary large cell carcinomas with different metastatic potential and to investigate the mechanism of the rarity of metastases in skeletal muscles. METHODS: Primary culture of newborn Wistar rat skeletal muscle cells was established, and the murine skeletal muscle conditioned medium(MMCM)was prepared to test its effect in vitro on pulmonary large cell carcinomas with different metastatic potential (PLA 801C with lower potential and PLA 801D with relatively higher potential) by MTT assay. Adriamycin was used as positive control for MMCM; murine benign renal cells BHK 21 were used as negative control for lung carcinoma cells. RESULTS: Proliferation of tumor cell lines of both PLA 801C and PLA 801D was significantly restrained when cultured with MMCM, while BHK 21 cells were not affected(P< 0.05). Compared with PLA 801C (significant only in primary MMCM), PLA 801D showed significantly decreased proliferation even when cultured in higher reciprocal of MMCM dilution(1/16 of primary MMCM). CONCLUSION: Skeletal muscle cells could selectively inhibit the proliferation of cancerous cells in vitro while benign cells are not affected. Tumor cells with higher metastatic potential are more sensitive to this effect.