室间隔缺损血清候选标志物锌指蛋白41的验证研究

Verification of ZNF41 as a serum candidate biomarker for ventricular septal defect

目的验证锌指蛋白41(zinc finger protein 41,ZNF41)是否为室间隔缺损(ventricular septal defect,VSD)患者的血清候选标志物。方法收集室间隔缺损患者的血清和与之性别、年龄、民族等频数相匹配的房间隔缺损(atrial septal defect,ASD)患者、法洛四联症(tetralogy of Fallot,TOF)患者和健康对照者的血清各20例,应用蛋白免疫印迹(Western blot)和酶联免疫吸附测定(enzyme-linked immunosorbent assay,ELISA)检测各组血清样本中锌指蛋白41的表达水平。结果 Western blot检测结果显示,锌指蛋白41在4组血清中均有表达,但VSD组中的相对表达水平均高于其他3组(均有P<0.05);ELISA检测结果可知VSD组的血清锌指蛋白41浓度为(136.72±56.44)pg/ml,ASD组为(94.54±41.98)pg/ml,TOF组为(100.69±37.08)pg/ml,健康对照组为(82.08±42.46)pg/ml,4组间差异有统计学意义(P=0.008),进一步两两比较,VSD组与其他3组之间差异均有统计学意义(均有P<0.05),而其他3组两两间差异均无统计学意义(均有P>0.05)。结论锌指蛋白41在VSD患者血清中表达水平较高,可能是VSD的血清候选标志物。

Objective To validate zinc finger protein 41( ZNF41) as a serum candidate biomarker in patients with ventricular septal defect( VSD). Methods The serum samples were obtained from patients with VSD,atrial septal defect( ASD),tetralogy of Fallot( TOF),and healthy controls. Each group had 20 study subjects. The four groups were frequency matched in age,gender,and nationality,respectively. The expression levels of ZNF41 in serum samples were detected by Western blot and enzyme-linked immunosorbent assay( ELISA). Results Western blot results showed that ZNF41 was detectable in all samples of study subjects. However,the relative expression level of ZNF41 in VSD group was higher than that in the other three groups( all P < 0. 05). The results of ELISA showed that the serum concentrations of ZNF41 in VSD,ASD,TOF and healthy group were( 136. 72 ± 56. 44) pg /ml,( 94. 54 ± 41. 98) pg /ml,( 100. 69 ±37. 08) pg /ml,and( 82. 08 ± 42. 46) pg /ml,respectively. There were statistically significant differences among the four groups( F = 4. 33,P = 0. 008). Given the further comparisons between each two groups,there was statistically significant difference between VSD group and the other three groups respectively( all P < 0. 05). Besides,there was no statistically significant difference between each two groups in the other three groups( all P > 0. 05). Conclusions The concentration of ZNF41 is higher in serum of patients with VSD,which suggests that ZNF41 may be used as a serum candidate biomarker for VSD.