ALDH2基因多态性与男性饮酒行为关系的分子流行病学研究

Associations between single nucleotide polymorphisms(SNPs) with acetaldehyde dehydrogenase gene 2 (ALDH2)and male drinking behavior

目的探讨乙醛脱氢酶基因(acetaldehyde dehydrogenase gene 2,ALDH2)多态性与男性饮酒行为的关系。方法采用Taq Man对823名正常男性ALDH2的9个标签单核苷酸多态性位点(single-nucleotide polymorphism,SNP)进行分型,使用Logistic回归分析基因型与饮酒行为的关系。结果调整年龄、受教育、婚姻、收入和吸烟后,5个SNP与饮酒行为的关联具有统计学意义(rs671(G>A),OR=0.35,95%CI:0.27~0.45,P<0.001;rs2283354(G>A),OR=1.61,95%CI:1.26~2.05,P<0.001;rs4767939(G>A),OR=1.46,95%CI:1.01~2.11,P=0.042;rs79073142(T>C),OR=1.71,95%CI:1.37~2.14,P<0.001;rs2106696(A>T),OR=1.75,95%CI:1.40~2.19,P<0.001)。携带危险等位基因个数越多,发生饮酒行为的可能性越高(趋势性P值<0.001)。SNP两两之间的交互效应没有统计学意义。结论 ALDH2基因多态性可影响中国男性饮酒行为。

Objective To investigate the associations of single nucleotide polymorphisms( SNPs) in acetaldehyde dehydrogenase gene 2( ALDH2) and male drinking behavior.Methods Taq Man was used for genotyping in 823 healthy men.Logistic regression was used to analyze the effects of the 9 tag SNPs on drinking behavior.Results Five SNPs were significantly associated with drinking behavior after adjusted by age,education,marriage,income and smoking status( rs671( G > A),OR = 0.35,95% CI:0.27-0.45,P < 0.001; rs2283354( G > A),OR = 1.61,95% CI:1.26-2.05,P <0.001; rs4767939( G > A),OR = 1.46,95% CI:1.01-2.11,P = 0.042; rs79073142( T > C),OR = 1.71,95% CI:1.37-2.14,P < 0.001 and rs2106696( A > T),OR = 1.75,95% CI:1.40-2.19,P < 0.001).Combined analysis showed that there was a significant dose-response relationship between the risk-allele numbers and high risk of drinking behavior( Ptrend< 0.001).Paired-interaction analyses of SNPs showed no significant effects.Conclusions SNPs in ALDH2 may influence drinking behavior in the Chinese male population.