山东省2011-2014年志贺氏菌分子分型及耐药性分析

Molecular typing and antibiotic resistance of Shigella isolated from 2011 to 2014 in Shandong Province

目的通过对山东省2011-2014年分离的志贺氏菌(Shigella)进行血清、毒力基因、分子分型和药敏等分析,了解山东省志贺氏菌的特性及流行趋势。方法用玻片凝集法进行血清分型,PCR方法扩增相关毒力基因,用脉冲场凝胶电泳技术进行分子分型,采用微量肉汤稀释法测定菌株抗生素敏感性。结果44株志贺氏菌主要为福氏志贺氏菌(Shigella flexneri)(54. 55%)和宋内志贺氏菌(Shigella sonnei)(43. 18%)。ipa H、set1、sen、ial毒力基因携带率分别为100%、43. 18%、56. 82%、50. 00%。经脉冲场凝胶电泳分型(pulsed field gel electrophoresis,PFGE),福氏志贺氏菌被分为18种带型,且总体相似度偏低;宋内志贺氏菌被分为14种带型,且89. 47%的菌株相似度在90%以上。44株志贺氏菌对15种抗生素中的14种均存在不同程度的耐药。93. 18%的菌株为多重耐药菌株。结论山东省志贺氏菌以福氏和宋内血清群为主,毒力基因携带率高,有聚类分布出现,耐药严重,应加强对志贺氏菌菌型、溯源和耐药性的监测。

Objective To study the characteristics and epidemic trend of Shigella in Shandong province through the analysis of serotype,virulence genes,molecular typing and drug sensitivity.Methods The serotype was classified using the method of slide agglutination.Polymerase chain reaction(PCR)was used to amplify the related virulence genes.The molecular typing was carried out by pulsed field gel electrophoresis(PFGE),and the antibiotic sensitivity of the strains was determined by microbroth dilution method.Results The main serogroups of 44 Shigella strains were Shigella flexneri(54.55%)and Shigella sonnei(43.18%).The carrying rates of ipaH,Set1,Sen and ial were 100%,43.18%,56.82%and 50.00%,respectively.By PFGE typing,the strains of Shigella flexneri were divided into 18 patterns with a low similarity.The strains of Shigella sonnei were divided into 14 patterns,and the similarity of 89.47%of the strains was more than 90%.44 strains of Shigella had different levels of resistance to 14 of the 15 antibiotics.93.18%of the strains were multidrug resistant.Conclusion The Shigella in Shandong province is dominated by serogroups of Shigella flexneri and Shigella sonnei,with high virulence gene carrying rate,clustering distribution and severe antibiotic resistance.It is necessary to strengthen the monitoring on serotype,traceability and antibiotic resistance of Shigella in Shandong province.