摘要
目的探讨miRNA-449a在人肺癌及癌旁组织中的表达差异及其临床意义。方法右江民族医学院附属医院2011-01-01—2013-06-30收治的肺癌患者58例,对照组为癌旁正常组织,病例组为鳞癌组织和腺癌组织,并根据miRNA-449a序列结构设计合成miRNA-449a模拟物,设10和20 mg/m L两个不同浓度,以0 mg/m L为阴性对照,用real-time PCR检测肺癌及其对应癌旁组织中miRNA-449a的表达;化学发光技术检测细胞内荧光素酶基因表达;四甲基偶氮唑蓝法检测细胞活性。结果鳞癌组和腺癌组的miRNA-449a的表达量为1.48±1.63和1.52±1.54,均显著低于癌旁正常对照组的2.74±1.55(P<0.01),且与瘤体大小有关(P<0.05)。10和20 mg/m L组细胞内的平均荧光强度为2 115±168和1 352±159,均显著低于阴性对照组的4 975±115(P<0.01),且抑制作用随浓度增加呈递增趋势。结论 miRNA-449a在肺癌组织中呈低表达,可下调细胞内荧光蛋白表达和诱导细胞凋亡。
Objective To investigate the expression and biological function of miRNA-449 a in lung cancer.Methods A case-control study was conducted in 58 patients diagnosed with lung cancer( carcinoma and adenocarcinoma) and normal tissue closely adjacent to tumor. MiRNA-449 a simulation was designed and synthesized,was dissolved into two different concentrations as 10 and 20 mg / m L. The expression of miRNA-449 a in lung cancer tissues and matched normal tissues were detected by Real time PCR. The expression of luciferase gene was detected by chemiluminescence technique. MiRNA-449 a mimics on cell apoptosis was evaluated by MTT assay.Results The mean tissues expression levels of miRNA-449 a in squamous carcinoma group and adenocarcinoma group were 1. 48 ± 1. 63 and 1. 52 ± 1. 54 respectively,and were significantly lower than in control group( 2. 74 ±1. 55)( P < 0. 01). The average intensity of fluorescent protein in 10 mg / m L group and 20 mg / m L group were2 115 ± 168 and 1 352 ± 159 respectively,and were significantly lower than that in control group( 4 975 ± 115)( P < 0. 01). Conclusions MiRNA-449 a was down-regulated expression in lung cancer and induced apoptosis.
出处
《基础医学与临床》
CSCD
2015年第1期44-47,共4页
Basic and Clinical Medicine
基金
广西自然科学基金(2011GXNSFA0198215)
