塞来昔布抑制人胰腺癌细胞系PANC-1的增殖、侵袭、迁移能力

Celecoxib inhibits proliferation,invasion and migration of human pancreatic cancer cell line PANC-1 in vitro

目的探讨COX-2抑制剂塞来昔布对人胰腺癌细胞系PANC-1增殖、侵袭、迁移能力的影响,确定塞来昔布作用的最佳浓度和最适宜的应用时间。方法不同浓度的塞来昔布(20、60和100μmol/L)处理胰腺癌细胞不同时间(24、48和72 h)后,用MTT比色法检测细胞的增殖能力;用Transwell实验检测细胞的侵袭能力;采用细胞划痕实验检测细胞的迁移能力。结果 MTT结果显示塞来昔布作用后胰腺癌细胞的增殖能力下降,呈时间和浓度依赖性(P<0.05);Transwell侵袭实验结果显示塞来昔布作用后胰腺癌细胞的侵袭能力下降,呈浓度依赖性(P<0.01);划痕实验结果显示塞来昔布作用后胰腺癌细胞的迁移能力下降,呈浓度依赖性(P<0.01)。结论塞来昔布以浓度梯度、时间梯度的形式减弱人胰腺癌细胞系PANC-1的增殖能力,以浓度梯度的形式减弱人胰腺癌细胞系PANC-1的侵袭及迁移能力。

Objective To investigate the effects of cyclooxygenase-2 inhibitor celecoxib on proliferation,invasion and migration of human pancreatic cancer cell line PANC-1 and then determine the optimal concentration of celecoxib and the most suitable application time. Methods Human pancreatic cancer cell line PANC-1 was treated with diverse concentrations of celecoxib( 20,60,100 μmol / L) for different durations( 24,48,72 h). Cell proliferation,invasion and migration capabilities were measured by MTT colorimetry,Transwell invasion assay,and scratch assay respectively. Results The proliferation capability of PANC-1 cell was reduced by celecoxib in a concentration- and time-dependent manner( P < 0. 05). In addition,the invasion and migration capabilities were decreased by celecoxib in a concentration-dependent manner( P < 0. 01). Conclusions Celecoxib attenuates theproliferation of human pancreatic cancer cell line PANC-1 in a concentration- and time-dependent manner. Celecoxib attenuates the invasion and migration in a concentration-dependent manner.