川芎嗪对实验性缺血—再灌注损伤心肌能量代谢的干预

Effect of ligustrazini on myocardial cell energy metabolism in the reperfusion injury after myocardial ischemia in rabbits

目的 探讨川芎嗪对心肌缺血—再灌注损伤 (MIRI)时心肌细胞能量代谢的影响。方法 实验兔 30只 ,随机分为正常对照组 (A组 )、MIRI组 (B组 )和MIRI+川芎嗪治疗组 (C组 )。复制MIRI模型 ,分别观察心肌组织内三磷酸腺苷 (ATP)、二磷酸腺苷 (ADP)、一磷酸腺苷 (AMP)含量、总腺苷酸量 (TAN)、能荷 (EC)及心肌超微结构的改变。结果 C组与B组比较 ,心肌组织内ATP、ADP、TAN含量及EC均明显增高〔ATP :C组为 (5 0 5± 1 34) μmol/g(湿重 ) ,B组为 (2 86± 1 2 2 ) μmol/g(湿重 ) ,P <0 0 1;ADP :C组为 (1 6 7± 0 4 0 ) μmol/g(湿重 ) ,B组为 (1 12± 0 2 5 ) μmol/g(湿重 ) ,P <0 0 1;TAN :C组为 (8 4 2± 1 90 ) μmol/g(湿重 ) ,B组为 (5 83± 1 5 5 ) μmol/g(湿重 ) ,P <0 0 1;EC :C组为 0 70± 0 0 3,B组为 0 5 8± 0 0 7,P <0 0 1〕 ;且与A组比较 ,ADP、AMP、TAN无明显差异〔ADP :A组为 (2 0 4± 0 5 3) μmol/g(湿重 ) ,P >0 0 5 ;AMP :A组为 (1 4 5± 0 2 9) μmol/g (湿重 ) ,P >0 0 5 ;TAN :A组为 (10 2 6± 2 0 5 ) μmol/g(湿重 ) ,P>0 0 5〕 ;心肌超微结构的异常改变显著减轻。结论 川芎嗪可改善缺血—再灌注损伤心肌的能量代谢。

Objective To study the effect of ligustrazini (LGT) on myocardial cell energy metabolism during myocardial ischemia-reperfusion injury (MIRI).Methods Using rabbit MIRI models,animals were randomly divided into three groups (n=10 in each),control group(A),MIRI group(B) and MIRI+LGT group(C),the contents of ATP,ADP and AMP (TAN,EC) in the myocardial tissue were measured,respectively.Meanwhile,the ultrastructure changes in myocardium were observed during MIRI.Results In C group ATP,ADP,TAN and EC levels of myocardial tissue were higher than those in B group(ATP:C(5 05±1 34) μmol/g(wet·weight),B(2 86±1 22) μmol/g(wet·weight),P<0 01;ADP:C 1 67±0 40,B 1 12±0 25,P<0 01;TAN:C 8 42±1 90,B 5 83±1 55,P<0 01;EC:C 0 70±0 03,B 0 58±0 07,P<0 01);and there were not significant differences between C and A group about ADP?AMP and TNA (ADP:A(2 04±0 53) μmol/g(wet·weight),P>0 05;AMP:A(1 45±0 29) μmol/g(wet·weight),P>0 05;TAN:A(10 26±2 05) μmol/g(wet·wt),P>0 05);abnormal changes of myocardial ultrastructure in B group were ameliorated remarkably during MIRI.Conclusions LGT could improve myocardial cell energy metabolism in the reperfusion injury after myocardial ischemia.