氮甲基左旋精氨酸对大鼠油酸型急性肺损伤时一氧化氮与丙二醛的影响及其保护作用的实验研究

Therapeutic effect of L-NMMA on experimental acute respiratory distress syndrome

目的 研究氮甲基左旋精氨酸 (N -momonethyi -L -arginine ,L -NMMA)对大鼠油酸型急性肺损伤 (ALI)时一氧化氮 (NitricOxide ,NO)与丙二醛 (Malondialdehyde ,MDA)的影响及其保护作用。方法 以 30只Wistar大鼠为实验动物 ,用油酸建立ALI的动物模型。把实验动物随机分为 3组 :正常组、损伤组和L -NMMA组 (以下简称为用药组 )。观察肺组织病理组织学改变、动脉血氧分压 (PaO2 )、肺水含量及动脉血浆中NO与MDA的变化。结果 用药组与损伤组比较 ,肺间质充血、出血、炎症、肺泡水肿明显减轻 ,而且未见透明膜形成 ;用药组与损伤组比较 ,动脉PaO2 明显提高 (P <0 0 1) ,而肺水含量明显减少(P <0 0 5 ) ;血浆中NO与MDA水平用药组与损伤组比较明显降低 (P <0 0 1)。结论 L -NMMA是通过抑制大量的NO和氧自由基的产生而对油酸型ALI起保护作用。

Objective To investigate the therapeutic effect of L-NMMA on experimental acute respiratory distress syndrome Methods 30 rats were divided into 3 groups:control group,oleic acid group and L-NMMA treated group Acute respiratory distress model was produced by intravenous injection of oleic acid Results PaO 2 in oleic acid group was evidently lower than that in normal grop Plasma NO,MDA and WW/DW in oleic acid group were markedly higher than that in normal group Compared with oleic acid group PaO 2 in L-NMMA group was slightly increased(P<0 05) and plasma NO,MDA and WW/DW in oleic acid group were significantly decreased(P<0 01) Histologic injury in L-NMMA treated group was significantly slighter than that in oleic acid group Conclusion L-NMMA might have a therapeutic effects on the oleic acid-induced acute respiratory distress syndrome