摘要
农杆菌介导的遗传转化技术是植物遗传转化中广泛采用的一种技术。如何提高转化率是植物遗传转化中的一个关键问题。已发现有许多物质能够促进农杆菌介导的遗传转化 ,如乙酰丁香酮 (AS)等许多酚类化合物都有助于农杆菌T -DNA的转移从而提高转化率。本实验在农杆菌转化大豆的过程中辅加化合物脯氨酸、硝酸银 ,通过对GUS基因表达的分析 ,结果表明这两种化合物均能明显地提高大豆的转化率 ,其中 2mg L浓度的脯氨酸及 2mg L的AgNO3 具有最佳效果 。
Agrobacterium tumefaciens has been used extensively for genetic engineering in soybean, but to date, transformation frequency of soybean is still low relative to that obtained in other plants. At an attempt to improve transformation efficiency, present experiment was conducted to add two compounds on transformation of cotyledonary nodes in soybean via Agrobacterium-mediation. The experiment result indicated that 2mg/L proline or 2mg/L AgNO 3 had the optimal effect on soybean transformation, the GUS gene tansformation rate was increased obviously.
出处
《大豆科学》
CAS
CSCD
北大核心
2003年第1期36-39,共4页
Soybean Science
基金
"国家转基因植物中试与产业化基地建设"专项基金资助项目 (J99-13 -0 0 1)
关键词
脯氨酸
硝酸银
农杆菌
大豆
遗传转化
Soybean
Agrobacterium tumefaciens
Genetic transformation
Proline
AgNO 3
