摘要
对生物技术在百合 (Liliumspp .)上的应用研究进展进行了综述 .生物技术在百合上的应用主要包括以下几个方面 :利用植物离体培养技术成功建立了百合快速繁殖体系和脱毒苗生产技术程序 ;利用胚胎拯救技术克服了百合杂交前后障碍获得百合新品种 ;蛋白质分子标记、DNA分子标记在百合的初步应用 ;通过农杆菌介导法、基因枪法、电激法等进行了百合的遗传转化 ,并通过基因枪法成功地获得了百合转基因的植株 .
The recent applications of biotechnology in lily ( Lilium spp.) were reviewed. A successful and efficient large scale propagation systems and a protocol to eliminate virus of lily have been established by means of tissue culture and rapid propagation. A system for embryo rescue has been developed to overcome prefertilization and postfertilization barriers,and new lily cultivars and hybrids have been obtained. Protein markers and DNA markers were applied widely in lily. Several gene transfer procedures have been initiated,and production of transgenic lily by micro projectile bombardment has recently been reported.
出处
《仲恺农业技术学院学报》
2002年第4期69-76,共8页
Journal of Zhongkai Agrotechnical College
关键词
生物技术
百合
应用
胚胎拯救
遗传转化
分子标记
lily ( Lilium spp.)
rapid propagation
embryo rescue
genetic transformation
molecular marker
