摘要
目的:观察咬合创伤时犬三叉神经节(TG)中神经生长因子受体TrkA mRNA及前速激肽原-A(PPTA)mRNA的表达变化,探讨咬合创伤导致口面痛的可能机制。方法:将高出咬合面1.5 mm的镍铬合金嵌体粘同于10只杂种犬右侧上颌第一、二磨牙咬合面的一类嵌体洞形内,造成对(牙合)牙的创伤。粘固嵌体后3、7、14、30、60天时取双侧TG。用反转录-聚合酶链式反应(RT-PCR)检测TG中TrRA及PPTA mRNA的表达变化并与无咬合创伤的对照组作比较。结果:(1)创伤后3-60天内创伤侧TG的TrkA和PPTA mRNA表达水平比对侧明显上调。(2)刨伤侧TrkA mRNA的表达量在3-60天内均高于对照组,7-30天内维持较高水平。(3)3-30天内PPTA mRNA水平明显高于对照组,3-14天达到最高,60天组与对照无差异。结论:咬合创伤能导致TG内TrkA mRNA及PPTA mRNA表达水平的上调。TrkA和PPTA可能参与了咬合创伤所导致的口面痛。
Objective: To investigate the possible mechanisms of orofacial pain induced by traumatic occlusion, the expression changes of TrkA and PPTA mRNAs were detected in the dog trigeminal ganglion (TG). Method: The occlusal surface of the first and the second maxillary right molars in 10 dogs were unilaterally raised 1.5mm with the casting Ni-Cr high inlay which was fixed in I Class inlay hole . On 3,7,14,30 and 60 days after teeth operation, TG of two sides were removed. The expression of TrkA mRNA and PPTA mRNA in TG were detected in both experimental and control groups with reverse transcription -polymerasa chain reaction (RT_PCR). Results : (1) Both TrkA and PPTA mRNAs expression in the TG of traumatic side were upregulated compared to the contralateral side during observation period (2) The TrkA mRNA expression in the TG of traumatic side was stronger than that in control group at all the time points ,with the higher expression levels during 7-30 days. (3) PPTA mRNA level was increased during 3-30 days and reached the peak level around 3-14 days after traumatic occlusion. No significant change was found between experimental group and control group at 60 days. Conclusion: The present results observed the upregulated expression of TrKA and PPTA mRNAs in dog TG after traumatic occlusion and suggested the possible involvement of TrKA and PPTA in the orofacial pain induced by traumatic occlusion.
出处
《口腔颌面修复学杂志》
2003年第1期4-7,共4页
Chinese Journal of Prosthodontics
基金
全军医药卫生科研基金(01MB081)
