国外粳型陆稻品系育性恢复基因等位性分析

ALLELIC ANALYSIS OF FERTILITY-RESTORING GENES CARRIED BY SOME FOREIGN JAPONICA UPLAND RICE VARIETIES

以新发现的6个粳型陆稻恢复系WAB450-11-1-3-P40-HB,WAB450-11-1-2-P61-HB,WAB450-1-I-P-91-HB,IRAT216,IRAT359,IRAT104及滇一型粳型水稻恢复系南29分别与滇一型不育系滇榆1号A杂交获得F1后再与保持系滇榆1号B回交形成的A/R//BBC1F1分离群体及F1与滇榆1号A回交的BC5F2分离群体为材料进行遗传研究,并用新发现的粳型陆稻恢复系与带恢复基因Rf1的C57杂交F1为父本,用滇榆1号A作母本测交进行恢复基因等位性测定,以花粉育性为主要指标,辅以小穗育性进行研究。结果表明:滇榆1号A与各恢复系的杂交F1花粉育性为50%左右,但结实率正常,为配子体不育类型,新发现的粳型陆稻恢复系WAB450-11-1-3-P40-HB,WAB450-11-1-2-P61-HB,WAB450-1-I-P-91-HB,IRAT216,IRAT359对滇榆1号A有正常恢复能力,而IRAT104仅能部分恢复;新恢复系WAB450-11-1-3-P40-HB,WAB450-11-1-2-P61-HB,WAB450-1-I-P-91-HB,IRAT216,IRAT359及云南粳型水稻恢复系南29的恢复性均由1对显性恢复基因控制,而IRAT104具有1对对滇一型细胞质雄性不育具部分恢复能力的恢复基因;新发现恢复系的恢复基因与著名粳稻恢复系C57所带的Rf1互为等位,IRAT104所携带的弱效恢复基因也与Rf1等位,为复等位基因,云南重要恢复系南29所带的恢复基因也与Rf1互为等位。

To study the fertility restoring ability of six newly found restoring lines for the Dian I type CMS line 'Dianyu 1A (CMS-D)', WAB450-11-1-3-P40-HB, WAB450-11-1-2-P61-HB, WAB450-11-1-I-P-91-HB, IRAT216, IRAT359, IRAT104 and Nan 29 were used to pollinate Diayu 1A to form F1 hybrids, which were then backcrossed with Dianyu 1B to form BC1F1 and with Dianyu 1A to form BC5F2 populations. They were also used to cross with C57, which carries the restoring gene Rf1, to form F1 hybrids, which were then test-crossed with Dianyu 1A to study their allelic relationship with Rf1. The results showed that the fertility ability of WAB450-11-1-3-P40-HB, WAB450-11-1-2-P61-HB, WAB450-11-1-I-P-91-HB, IRAT216, IRAT359 and Nan 29 is controlled by a dominant gene, which is allelic to Rf1, and the partial fertility ability of IRAT104 is controlled by another dominant gene, which is also allelic to Rf1.