摘要
[目的]对福建省分离的80株E.coli O157:H7菌株进行毒力及特征基因的检测分析。[方法]应用聚合酶链反应(PCR),以志贺毒素基因(stx)、粘附抹平因子基因(eaeA)、溶血素基因(hlyA)、O157抗原编码基因(rfbO)157)和H7抗原编码基因(fliCH7)为靶基因进行检测。[结果]福建省分离的O157:H7菌株毒力基因携带率为6.25％(5/80)。菌株毒力基因图谱为stx+eaeA+hlyA和eaeA+hlyA。rfbO157基因阳性与O157血清型符合率为100％。[结论]福建省分离的O157:H7菌株毒力基因携带率(6.25％)低于江苏省疾病高发地区(85.7％,P<0.05)。
[Objective] To detect and analyze the toxic and characteristic gene of O157 : H7 isolated from Fujian. [Methods] To detect shiga toxin gene (stx), intimin gene (eaeA), hemolysin gene (hlyA) encoding gene of O157 antigen (rfb O157) and encoding gene of H7 antigen (fliC H7) by the method of polymerase chain reactions (PCR). [Results] The carrying rate of toxic gene of O157 : H7 was 6. 25% (5/80). The toxic gene fingerprints were stx+eaeA + hlyA and eaeA + hlyA. The coincidence rate among rfb O157 gene with O157 serotype was 100%. [Conclusions] The carrying rate of toxic gene of O157 : H7 in Fujian (6. 25%) is lower than that in the high incidence area of Jiangshu province (85.7%, P<0. 05).
出处
《海峡预防医学杂志》
CAS
2003年第1期1-3,共3页
Strait Journal of Preventive Medicine
基金
福建省卫生厅青年科研基金资助(项目号:Y2K-1-16)
