消化道癌的傅立叶变换红外光谱研究

FR-IR study on cancer of digestive tract

目的 研究消化道癌的傅立叶变换红外光谱 (fouriertransforminfraredspectroscopy ,FT -IR)表现。方法 应用FT -IR对 12 1例消化道癌及癌旁正常组织进行检测 ,结合常规病理结果指认、总结不同组织的光谱特征。结果 癌和癌旁正常组织具有不同的FT -IR光谱表现 ,15 5 0cm- 1 处的酰胺Ⅱ带在癌组织中显得较弱 ,峰形低平 ,而在正常组织中则较强 ,峰形高尖。 10 80cm- 1 处核酸的吸收谱带在癌组织中较强。正常组织中 14 5 0cm- 1 处的峰多强于 14 0 0cm- 1 处 ,而在癌组织中则相反。结论 消化道癌及癌旁正常组织的FT -IR谱图不同 ,主要表现为组织中蛋白质。

Objective To investigate the special FT-IR spectra of cancer of digestive tract. Methods Cancer tissues and the normal tissues of digestive tract were studied by FT-IR and HE stain, the special spectra data with different tissues were summarized. Results Different spectra were found in normal and cancer tissues. The peak at 1 550 cm -1 was weak and wide in cancer tissues but strong and high in normal tissues. The peak at 1 080 cm -1 was stronger in cancer tissues than in normal tissues. The peak at 1 450 cm -1 was higher than that at 1 400 cm -1 in normal tissues and lower than that at 1 400 cm -1 in cancer tissues. Conclusions The normal and cancer tissues of digestive tract have different FT-IR spectra, which were mainly caused by the changes of protein, nucleic acid and phospholipid.