大肠癌患者外周血细胞角蛋白-20信使核糖核酸与白细胞介素-10联合检测的临床意义

THE CLINICAL SIGNIFICANCE OF THE DETECTION OF CK-20mRNA AND IL-10 IN PERIPHERAL BLOOD OF PATIENTS WITH COLORECTAL CANCER

目的 联合检测大肠癌患者外周血细胞角蛋白 - 2 0信使核糖核酸 (CK - 2 0mRNA)与血浆中白细胞介素 - 1 0 (IL - 1 0 ) ,并评价其临床意义。方法 应用RT -PCR检测大肠癌患者外周血CK - 2 0mRNA ;应用ELISA检测其血浆IL - 1 0浓度。结果 对照组2 5例健康查体者 ,其外周血中未见CK - 2 0mRNA的表达 ;观察组 46例大肠癌患者 ,2 5例外周血中CK - 2 0mRNA表达阳性。大肠癌患者外周血CK - 2 0mRNA的表达与Dukes分期关系为 :A期 2 0 .0 % (1 / 5)、B期 2 7.3 % (3/ 1 1 )、C期 61 .1 % (1 1 / 1 8)、D期 83 .3(1 0 /1 2 ) ;其中C、D期与A、B期比较差异有高度显著性 (P <0 .0 1 )。大肠癌患者血浆IL - 1 0浓度明显高于健康查体者 ,且与Dukes分期显著相关。外周血CK - 2 0mRNA阳性表达的大肠癌患者血浆IL - 1 0水平高于阴性者。结论 联合检测大肠癌患者外周血CK -2 0mRNA与IL - 1 0 ,掌握检测大肠癌转移的相关指标 ,可以及时全面地了解大肠癌患者病情进展情况。

Objective To evaluate the clinical significance of CK-20mRNA and IL-10 in patients with Colorectal Cancer. Methods CK-20mRNA in peripheral blood was detected by Reverse Transcription-Polymerase chain reaction and IL-10 by ELISA. Results The expression of CK-20mRNA was negative in peripheral blood of 12 healthy volunteers.Instead,CK-20mRNA was positive in peripheral blood of 25 out of 46 patients with colorectal cancer.The positive rate of the expression of CK-20mRNA was 20.0%(1/5) in clinical stage A,27.3%(3/11) in B,61.1%(11/18)in C,83.3%(10/12) in D. In clinical stage C and D,the positive rate of the expression of CK-20mRNA was significantly different with that in A and B. The plasma IL-10 level of patients with colorectal cancer was much higher than that of healthy volunteers,and it has significantly relevant with Dukes stage. Conclusion CK-20mRNA and IL-10 are useful indicators to the early metastasis of colorectal cancer, meanwhile they can also monitor the clinical therapy efficiency.